The super-resolution microscope Elyra 7, equipped with Structured Illumination Microscopy (SIM), doubles the conventional confocal resolution and allows for the discrimination of the finest sub-organelle structures in tissues, bacteria, organoids, neurons, and both living and fixed cells, even when structures are no more than 100 nm apart. You do not need to compromise on resolution when imaging at high speeds, thanks to the minimal exposure required for live observations.

The ZEISS Elyra 7 offers a range of techniques to meet your experimental needs across various scales, optimally matching resolution, speed, and sensitivity requirements to your demanding samples. Utilize SIM Apotome for fast optical sectioning and Lattice SIM for super-resolution imaging.

Additionally, Single-Molecule Localization Microscopy (SMLM) and Total Internal Reflection Fluorescence (TIRF) techniques enable investigations at the molecular level. These techniques can be combined to enhance the insights gained from your specimens and correlate the acquired data effectively.

The Elyra 7 allows you to integrate super-resolution and high-dynamic imaging without the need for special sample preparation or extensive knowledge of complex microscopy techniques. The new Apotome mode in Elyra 7 provides ultra-fast optical sectioning of 3D samples, while its groundbreaking light efficiency facilitates gentle, super-resolution imaging at an impressive speed of 255 frames per second, producing data faster than ever before.

Sample Carriers

The sample carriers may include live cell imaging dishes, Multichamber , and fix slides

Applications

1. SIM: Super-resolution Imaging:

SIM (Structured Illumination Microscopy): This mode utilizes a patterned light source to improve resolution beyond the diffraction limit of conventional microscopy. It allows for three-dimensional imaging with enhanced detail.

2. Single Molecule Localization Microscopy (3D Imaging at Molecular Resolution)

PALM (Photo-activated Localization Microscopy) and STORM (Stochastic Optical Reconstruction Microscopy): These are single-molecule localization techniques that provide super-resolution images by precisely locating individual fluorescent molecules over time.

3. SIM Apotome:

Live cell imaging with a widefield system often suffers from out-of-focus blur or background signal. These effects can decrease contrast and resolution of your images. The SIM Apotome acquisition mode of Elyra 7 uses structured illumination to give you fast optical sectioning with crisp contrast and high lateral and axial resolution.

4. Total Internal Reflection Fluorescence (TIRF):

TIRF microscopy is a highly effective imaging technique that allows researchers to investigate biological processes at the cell membrane with exceptional clarity. By utilizing total internal reflection, it selectively excites fluorescent molecules close to the glass-water interface, significantly reducing background noise. This method is essential for studying dynamic cellular interactions and provides insights into the behavior of individual biomolecules in their native environments.

5. Antibody conjugates and labels:

Conjugates of antibodies for fixed-cell applications and proteins, dextrans, or other biomolecules for fixed or live cells.

6. Live cell imaging:

Live-cell labeling with fluorescent proteins: Useful for thicker sections and live-cell imaging, targeted intracellular labels.

7. Multi-color Imaging:

The Elyra 7 supports simultaneous imaging of multiple fluorescent labels, enabling the study of complex biological interactions.

8. High-content screening:

Quickly acquire high-resolution images of multi-labeled cell cultures from various sample carriers, such as multi chambers, Petri dishes, fixed slides, etc.