Titan Krios (Thermofisher) cryo-electron microscope is an atomic resolution cryo-EM instrument with enhanced productivity, equipped with a Bioquantum K3 detector and energy filter (Amtek-Gatan). Along with the microscope, the facility has all accessories for frozen-hydrated sample preparation for single particle reconstruction and cryotomography. The facility can collect high-resolution data on biomolecules like proteins, protein complexes, viruses, nanoparticles, etc., and provide detailed information about cell and tissue sections.
1. Suitable for single particle analysis, electron tomography and micro electron diffraction.
2. The Cryo function makes it fully applicable in medicine, biology, pharmacy and studying unknown materials.
3. 3D analysis and imaging.
4. Analysis at a molecular level.
5. Structural-based drug designing.
Instrument Make |
Thermofisher Krios G3 |
Imaging |
Extreme high-brightness field emission gun (X-FEG) and Flexible accelerating voltage from 80-300 kV with the automatic condenser, objective, and SA apertures Aberration-free image shift (AFIS) between grid holes for shorter relaxation times Fringe-free imaging (FFI) for multiple image acquisitions per grid hole |
Sample loading |
Cryo-Autoloader for automated and contamination-free loading of cassettes containing up to 12 Autogrids |
Detectors |
Thermo Scientific Falcon 3 Direct Electron Detector |
Condensers and lenses |
Three-condenser lens system for automated, continuous parallel sample illumination Symmetric constant power C-TWIN objective lens with wide-gap pole piece (11 mm) with mode switching (imaging modes and diffraction) facility. Computerized 4-axis specimen stage with ±70-degree alpha tilt |
Advanced Performance Monitoring |
self-assessment of microscope status, combined with automated alignments, ensures optimal experimental conditions |
Software |
Low-dose software suite for minimized electron dose during cryo-TEM operation, Tomography, EPU, Inspect 3D |
Additional Features |
Gatan Bio Quantum Energy Filter |
Lab Location :
Ground Floor B 99,
IIT Delhi, Hauz Khas,
New Delhi - 110016
Operating Scientist:
Mr. Abhishek Trivedi
Email : kriosiitd@gmail.com
Professor In charge:
Prof. Manidipa Banerjee
Kusuma School of Biological Sciences
IIT Delhi, Hauz Khas, New Delhi - 110016