Titan Krios G3i (Thermo Fisher Scientific) is a state-of-the-art 300 kV cryo-electron microscope capable of delivering near-atomic resolution 3D structures of proteins, viruses, biomolecules, and cellular assemblies in their native frozen state. It enables high-throughput single-particle analysis and cryo-electron tomography, helping industries accelerate structure-based drug discovery, vaccine development, and biologics research. Equipped with advanced automation, direct electron detectors, and energy filters, the system provides exceptional image quality, stability, and rapid data collection for complex biological samples. For pharmaceutical, biotechnology, and life science industries, the Titan Krios G3i significantly reduces timelines for understanding molecular mechanisms, identifying therapeutic targets, and advancing next-generation therapeutics.

Key Instrument Features

• 300 kV high-brightness Field Emission Gun (FEG)
• Fully automated cryogenic sample loading system
• High mechanical and thermal stability
• Automated microscope alignments
• Low-dose imaging for radiation-sensitive specimens
• Direct electron detector for high-resolution data acquisition
• Energy filter for enhanced image contrast
• Automated high-throughput data collection
• Optimized for SPA and Cryo-ET workflows
• Remote monitoring and automated imaging capabilities

Sample Requirements

To ensure successful data collection, samples should satisfy the following criteria:

• Properly vitrified cryo-EM grids
• Uniform vitreous ice
• Minimal crystalline ice contamination
• Appropriate particle concentration
• Biochemically homogeneous samples
• Stable sample preparation and storage
• Cryogenic transport in suitable shipping containers

Detailed sample preparation guidelines are available from the facility upon request (kriosiitd@gmail.com).

Available Services

The facility provides the following services:

• Cryo-TEM Image Acquisition - Automated high-resolution image acquisition for vitrified biological specimens.
• Single Particle Analysis (SPA) - Data collection for three-dimensional reconstruction of purified macromolecular complexes.
• Cryo-Electron Tomography (Cryo-ET) - Tilt-series acquisition for structural analysis of cellular specimens and biological assemblies in their native environment.
• Screening and Optimization - Assessment of vitrified grids for ice quality, particle distribution, and imaging suitability.
• User Training - Hands-on training for qualified users in microscope operation, experiment planning, and data acquisition.
• Data Management - Support for data transfer, storage, and guidance on downstream image processing workflows.

Applications

The Titan Krios G3i supports a broad range of research applications, including:

• High-resolution structure determination of proteins and protein complexes
• Structural analysis of viruses and virus-host interactions
• Membrane protein characterization
• Cryo-electron tomography of cells and tissues
• In situ structural biology
• Drug target identification and structure-based drug discovery
• Therapeutic antibody and biologics development
• Vaccine antigen characterization
• Structure determination of dynamic and heterogeneous macromolecular assemblies
• Biomolecular interaction and conformational studies